Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-60
... cleotides by the modified triester method . Methods Enzymol . 65 : 610 . Newgard , C.B. , K. Nakano , P.K. Hwang ... cleotide primer . Proc . Natl . Acad . Sci . 78 : 616 . Stawinski , J. , T. Hozumi , S.A. Narang , C.P. Bahl , and R ...
... cleotides by the modified triester method . Methods Enzymol . 65 : 610 . Newgard , C.B. , K. Nakano , P.K. Hwang ... cleotide primer . Proc . Natl . Acad . Sci . 78 : 616 . Stawinski , J. , T. Hozumi , S.A. Narang , C.P. Bahl , and R ...
Page 15-55
... cleotide . • The difference in thermal stability between perfectly matched hybrids and mismatched hybrids is sufficiently great that the mutagenic oligonu- cleotide can be used to screen bacteriophage M13 plaques by hybridiza- tion for ...
... cleotide . • The difference in thermal stability between perfectly matched hybrids and mismatched hybrids is sufficiently great that the mutagenic oligonu- cleotide can be used to screen bacteriophage M13 plaques by hybridiza- tion for ...
Page 15-112
... cleotide - directed mutagenesis . Com- ments ( United States Biochemical Corp. ) 15 ( 2 ) : 23 . Schold , M. , A. Colombero , A.A. Reyes , and R.B. Wallace . 1984. Oligonu- cleotide - directed mutagenesis using plasmid DNA templates and ...
... cleotide - directed mutagenesis . Com- ments ( United States Biochemical Corp. ) 15 ( 2 ) : 23 . Schold , M. , A. Colombero , A.A. Reyes , and R.B. Wallace . 1984. Oligonu- cleotide - directed mutagenesis using plasmid DNA templates and ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml