Molecular Cloning: A Laboratory Manual, Book 2 |
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Page xix
... Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF cDNA PROBES 10.38 Identification of cDNA Clones 10.38 DIFFERENTIAL SCREENING 10.38 ABSORBED PROBES ...
... Synthesis of RNA In Vitro 10.32 Synthesis of RNA Probes Radiolabeled to High Specific Activity 10.34 Troubleshooting 10.36 SYNTHESIS OF cDNA PROBES 10.38 Identification of cDNA Clones 10.38 DIFFERENTIAL SCREENING 10.38 ABSORBED PROBES ...
Page 8-12
... synthesis , there is a competition between degradation of the mRNA and initiation of DNA synthesis . In addition , RNAase H can cleave the RNA template near the 3 ' - hydroxyl terminus of the growing DNA strand if the polymerase pauses ...
... synthesis , there is a competition between degradation of the mRNA and initiation of DNA synthesis . In addition , RNAase H can cleave the RNA template near the 3 ' - hydroxyl terminus of the growing DNA strand if the polymerase pauses ...
Page 8-29
... synthesis of the second strand . This reaction is far less efficient than replacement synthesis of the second strand . Second , the method generates cDNA libraries in plasmid vectors , which are more difficult to maintain and screen ...
... synthesis of the second strand . This reaction is far less efficient than replacement synthesis of the second strand . Second , the method generates cDNA libraries in plasmid vectors , which are more difficult to maintain and screen ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml