Molecular Biology Labfax, Volume 1T. A. Brown Labfax volumes are purpose-designed data reference books for practicing scientists. Each book presents key information for a major subject in one place and therefore saves hours of searching. The authors and editors of each Labfax volume have searched the original literature for the accurate data which they know the specialist needs.
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Contents
NUCLEIC ACID BLOTTING AND HYBRIDIZATION | 1 |
Melting temperatures for oligonucleotides of different lengths and base compositions Table | 7 |
Detection of radioactivity | 9 |
Typical hybridization temperatures for different applications Table | 13 |
Troubleshooting | 15 |
Protection from radiochemical hazards | 16 |
Phenotypes of E coli loci and genes relevant to recombinant DNA experiments Table | 17 |
Properties of E coli deletions relevant to recombinant DNA experiments Table | 23 |
AGAROSE GEL ELECTROPHORESIS | 183 |
Ribonuclease U2 | 190 |
Chain termination DNA sequencing | 196 |
T4 DNA ligase | 203 |
SAFETY | 221 |
References | 223 |
Agarose gels | 225 |
GENOMES | 233 |
Bacteriophage 2 | 29 |
Buffers for working with phage 2 Table | 36 |
References | 41 |
Restriction in the laboratory | 73 |
Effects of DNA methylation on restriction | 98 |
References | 122 |
Details of individual enzymes and proteins | 132 |
Calculation of the relative centrifugal force | 133 |
POLYMERASE CHAIN REACTION | 136 |
Care of rotors | 140 |
60 | 142 |
Details of individual compounds | 150 |
BisMSB | 158 |
Micrococcal DNA polymerase | 164 |
ddTTP | 171 |
Polynucleotide phosphorylase | 177 |
DNA SEQUENCING | 179 |
Amounts of doublestranded DNA of different lengths corresponding to 3 x 105 molecules | 239 |
Electrophoresis systems | 240 |
Cloning PCR products | 245 |
The genetic code | 247 |
Animals | 269 |
Mitochondria | 277 |
CLONING VECTORS | 291 |
Sequencing PCR products | 292 |
References | 300 |
pGEM11Zf+ | 311 |
pNEB193 | 326 |
Labelling procedures | 358 |
M13 cloning vectors | 363 |
77 | 364 |
| 374 | |
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Common terms and phrases
Acad Acids Res ATCGAT BamHI Biol biosynthesis Blunt Buffer CCGCGG CCGG CCSGG CCWGG CGATCG Cloning sites Cloning Vectors codons codons UUU coli content of genes CTAG CTCGAG CTGCAG CYCGRG DNA ligase DNA polymerase dsDNA EcoR EcoRI endAl Endodeoxyribonuclease Endoribonuclease England Biolabs Enzyme Isoschizomers Recognition exonuclease Frequencies per thousand GATC GC content GC frequency genes containing genome GGATCC GGCC GGNCC GGTACC GGTCTC GGWCC GRGCYC HindIII KpnI lacZ lacZAM15 mcrA MgCl2 ml¹ NaCl Natl nd nd nd Nucl nucleotide overhang CG phage Phagemid plasmid Polylinker Proc Promega protein PstI Reaction conditions Recognition sequence recombinant relAl Replicator pMB1 Restriction/Methylation reverse transcriptase Ribonuclease Sall second nucleotide sequenceb,c Smal ssDNA thousand codons Tris-HCl pH TTCGAA XbaI XhoI Туре