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Molecular cloning:

a laboratory manual, Volume 3
Front Cover
4 Reviews
Cold Spring Harbor Laboratory, 1989 - Eukaryotic cells

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Review: Molecular Cloning: A Laboratory Manual

User Review  - Adrianna - Goodreads

Important book for someone in molecular biology field Read full review

Review: Molecular Cloning: A Laboratory Manual

User Review  - Sofia - Goodreads

This is the closest thing a molecular biologist has to Holy Scripture. There isn't a single molbio lab in the world that doesn't worship at the altar of Saint Sambrook, patron of DNA, RNA and protein. Goodreads needs a new rating system for this, because five stars isn't enough. Read full review

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Contents

BOOK
16-2
BOOK
16-3
Hybridization 11
16-11
Copyright

72 other sections not shown

Common terms and phrases

acetate acrylamide activity Adjust the volume aliquots amino acids ammonium persulfate amount antigen antisera aqueous assay autoclaving bacteria bacteriophage T7 binding bovine serum albumin cDNA cell lines cloned gene coli column culture deionized H2O denatured described on pages dilution Dissolve dithiothreitol DNA polymerase EDTA EDTA pH 8.0 efficiency elution Escherichia coli ethanol eukaryotic expression extract foreign protein fragment glycerol hybridization immunoglobulin immunoprecipitation Incubate lysate lysis buffer mammalian cells medium method mg/ml microfuge tube minutes at 4°C minutes at room mixture monoclonal antibodies mRNA NaCl Natl nitrocellulose nitrocellulose filter nucleic acid pellet phosphate plasmid plate PMSF polyclonal precipitate prepared promoter protein of interest radioactive radiolabeled reaction reagent Remove restriction enzyme Resuspend room temperature sample SDS gel-loading buffer SDS-polyacrylamide gel sequence sodium sodium azide sterile stock solution strain supernatant target antigen target protein termini transcription transfection Transfer Tris Cl pH vectors wash western blotting

Bibliographic information

QR code for Molecular cloning
TitleMolecular cloning: a laboratory manual, Volume 3
Molecular Cloning: A Laboratory Manual, E. F. Fritsch, ISBN 0879693096, 9780879693091
AuthorsJoseph Sambrook, E. F. Fritsch, Tom Maniatis
Edition2
PublisherCold Spring Harbor Laboratory, 1989
Original fromthe University of Michigan
Digitized23 Feb 2010
ISBN0879693096, 9780879693091
SubjectsEukaryotic cells
Molecular cloning
  
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