Gene Cloning: An IntroductionThird edition. This concise and accessible introduction to gene cloning assumes almost no background knowledge. |
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Page 50
... Polynucleotide kinase , which attaches 5 ' - phosphate groups . ( c ) Terminal deoxynucleotidyl trans- ferase , which attaches nucleotides to the 3 ' - termini of polynucleotides in either ( i ) single - stranded or ( ii ) double ...
... Polynucleotide kinase , which attaches 5 ' - phosphate groups . ( c ) Terminal deoxynucleotidyl trans- ferase , which attaches nucleotides to the 3 ' - termini of polynucleotides in either ( i ) single - stranded or ( ii ) double ...
Page 70
... polynucleotide strand showing the chemical distinction between the 5 ' - P and 3 ' - OH termini Base Base شقيقب One nucleotide Base ( b ) In the double helix the polynucleotide strands are antiparallel 5 ' 3 ' ← 3 ' 5 ' ( c ) Ligation ...
... polynucleotide strand showing the chemical distinction between the 5 ' - P and 3 ' - OH termini Base Base شقيقب One nucleotide Base ( b ) In the double helix the polynucleotide strands are antiparallel 5 ' 3 ' ← 3 ' 5 ' ( c ) Ligation ...
Page 224
... polynucleotide ( or region of a polynucleotide ) able to direct synthesis of a complementary polynucleotide . Terminator The short nucleotide sequence downstream of a gene that acts as a signal for termination of transcription . 5 ...
... polynucleotide ( or region of a polynucleotide ) able to direct synthesis of a complementary polynucleotide . Terminator The short nucleotide sequence downstream of a gene that acts as a signal for termination of transcription . 5 ...
Contents
Preface | 1 |
plasmids and bacteriophages | 13 |
Purification of DNA from living cells | 25 |
Copyright | |
12 other sections not shown
Common terms and phrases
Adaptors agar amino acid ampicillin animal antibiotic antibody B-galactosidase bacterial cells bacteriophage bacterium BamHI base pairing biotechnology blunt ended carried cDNA cell extract centrifugation Chapter cleavage cleave cloned gene cloning vectors coded coli coli cells colonies contain copy number cosmid culture density digestion DNA fragments DNA ligase DNA sequencing double-stranded EcoRI electrophoresis enzyme expression vector Figure gene cloning gene cloning experiment host cell hybridization probing insulin intron labelled lacZ leu2 ligation lysed medium method mitochondrial Molecular Biology mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide organisms packaging phage DNA phage particles plant cells plaques plasmid DNA polylinker polymerase polynucleotide preparation produced promoter pUC8 purified recognition sequences recombinant DNA recombinant DNA molecule recombinant protein replication restriction endonucleases restriction sites resulting segment specific sticky ends strand structure synthesis T-DNA techniques tetracycline Ti plasmid transformed translation product trpA types unique restriction virus vitro yeast zein