Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 20
... Thus all cells in a colony are identical ; they are members of a clone . The ability to obtain individual colonies is important because gene cloners cannot visually distinguish one gene from another . We cannot simply use forceps to ...
... Thus all cells in a colony are identical ; they are members of a clone . The ability to obtain individual colonies is important because gene cloners cannot visually distinguish one gene from another . We cannot simply use forceps to ...
Page 88
... thus , they can sometimes survive for many years outside their host cell . A virus that infects a bacterium is called a bacteriophage or simply a phage . It is possible to cut plasmid and bacteriophage DNA in a specific place , insert a ...
... thus , they can sometimes survive for many years outside their host cell . A virus that infects a bacterium is called a bacteriophage or simply a phage . It is possible to cut plasmid and bacteriophage DNA in a specific place , insert a ...
Page 173
... Thus there are about 24,000 combi- nations ( 80 X 50 × 6 ) that can form . Flexibility in the V / D and the D / Jjunctions probably adds 100 more ways to combine the genes , so the total number of heavy chain combinations is about 2.4 ...
... Thus there are about 24,000 combi- nations ( 80 X 50 × 6 ) that can form . Flexibility in the V / D and the D / Jjunctions probably adds 100 more ways to combine the genes , so the total number of heavy chain combinations is about 2.4 ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses