Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 127
... cDNA Plasmid DNA ( confers antibiotic Rabbit DNA ( whole genome ) resistance ) cDNA plasmid Fragmented rabbit DNA Select and amplify cDNA plasmid Bacteria ( antibiotic- sensitive ) 000 Cut and radioactively label cDNA plasmid Many copies of ...
... cDNA Plasmid DNA ( confers antibiotic Rabbit DNA ( whole genome ) resistance ) cDNA plasmid Fragmented rabbit DNA Select and amplify cDNA plasmid Bacteria ( antibiotic- sensitive ) 000 Cut and radioactively label cDNA plasmid Many copies of ...
Page 131
... cDNA . It was decided to first clone the cDNA into a plasmid . Then large amounts of it could be obtained easily from bacterial cells . This cloned cDNA would be radioactively labeled and used to test the phage plaques for hemoglobin ...
... cDNA . It was decided to first clone the cDNA into a plasmid . Then large amounts of it could be obtained easily from bacterial cells . This cloned cDNA would be radioactively labeled and used to test the phage plaques for hemoglobin ...
Page 138
... cDNA clones derived from mRNA . This distinction is important , for as described in Chapter 10 , mRNA from higher organisms often contains only parts of the nucleotide sequence of the gene . Thus cDNA clones would also contain only ...
... cDNA clones derived from mRNA . This distinction is important , for as described in Chapter 10 , mRNA from higher organisms often contains only parts of the nucleotide sequence of the gene . Thus cDNA clones would also contain only ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses