Understanding DNA and Gene Cloning: A Guide for the CuriousGene cloning technologies continue to spur advances in many biological disciplines. Intended for the non-scientist who is interested in gaining sufficient background to understand often complex articles, this text aims to serve as a supplement for general biology or introductory genetics courses. It is also suitable as a main text in short courses for non-science majors interested in current topics such as AIDS, biotechnology, and bioethics. The previous edition of this book was published in 1984. |
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Page 128
... DNA . It also inactivates nucleases present in the extract that might otherwise begin cutting the DNA . Next an oily ... rabbit DNA in the ly- sate was further purified by centrifugation in a density gradient as de- scribed in Chapter 6 ...
... DNA . It also inactivates nucleases present in the extract that might otherwise begin cutting the DNA . Next an oily ... rabbit DNA in the ly- sate was further purified by centrifugation in a density gradient as de- scribed in Chapter 6 ...
Page 131
... DNA fragments from the organism being examined ( in our case rabbit hemoglobin cDNA ) are inserted into plasmid DNA using re- striction endonucleases and ligase , the DNA mixture is added to E. coli cells , and some of the DNA molecules ...
... DNA fragments from the organism being examined ( in our case rabbit hemoglobin cDNA ) are inserted into plasmid DNA using re- striction endonucleases and ligase , the DNA mixture is added to E. coli cells , and some of the DNA molecules ...
Page 132
... rabbit DNA from those that do NOT have rabbit DNA attached to them ( Figure 8-2 ) . To execute this trick one uses a cloning vehicle , a plasmid , with two genes for antibiotic resistance . Often one gene is for tetracycline resistance ...
... rabbit DNA from those that do NOT have rabbit DNA attached to them ( Figure 8-2 ) . To execute this trick one uses a cloning vehicle , a plasmid , with two genes for antibiotic resistance . Often one gene is for tetracycline resistance ...
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Common terms and phrases
agar plate amino acids antibody antigen atoms bacterial cells bacterial colonies bacterial DNA bacteriophage bacterium base pairs bind biologists called carbon cDNA cellular centrifuge Chapter chemical reactions chromosome cloned genes cloning vehicle codon coli cells complementary base pairing cules culture disease DNA molecules DNA polymerase DNA replication DNA strands double-stranded encoded enzyme ERSITY Figure film gel electrophoresis gene cloning gene expression genetic engineering genetic information hemoglobin hemoglobin genes host hybridization hydrogen infection inserted introns joined lambda ligase messenger RNA molecular mRNA mutation nucleic acid nucleotide pairs nucleotide sequence occurs organisms particular phage DNA phage particles piece of DNA plaques plasmid DNA primer produce protein purified rabbit DNA radioactive probe recombinant DNA region of DNA repressor restriction endonuclease retroviruses reverse transcriptase ribosome ribozyme SAN DIEGO Scientific American single-stranded splicing structure subunits sugar tein test tube tion transcription transfer RNA transposon tumor virus viruses