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Review: Molecular Cloning: A Laboratory ManualUser Review - Adrianna - Goodreads
Important book for someone in molecular biology field Read full review
Review: Molecular Cloning: A Laboratory ManualUser Review - Sofia - Goodreads
This is the closest thing a molecular biologist has to Holy Scripture. There isn't a single molbio lab in the world that doesn't worship at the altar of Saint Sambrook, patron of DNA, RNA and protein. Goodreads needs a new rating system for this, because five stars isn't enough. Read full review
Acad activity aliquots alkaline phosphatase analysis antibody antigen assay bacterial bacteriophage T4 baculovirus binding Biochem Biol buffer cDNA cell lines centrifugation chemical fume hood chemiluminescent cleavage cloning codon concentration containing culture detection DNA polymerase DNase efficiency electrophoresis electroporation encoding enzyme Escherichia coli ethanol ethidium bromide exonuclease filter fluorescent fragments fume hood fusion protein gene expression genomic gloves and safety green fluorescent protein harmful by inhalation hybridization Incubate ingestion interactions labeled luciferase lysate mammalian cells medium membrane method microarray microfuge minutes molecular molecules mutations Natl nuclear Nucleic Acids Nucleic Acids Res oligonucleotides p-galactosidase peptide phage phosphatase phosphate plasmid plates probe Proc promoter protocol purification radiolabeled reaction reagents restriction enzyme room temperature safety glasses sample screening sensitive sequence skin absorption sodium specific staining stock solutions substrate target protein tetracycline tion transcription transfection tube two-hybrid vectors Wear appropriate gloves yeast
Page 12-20 - MJ: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
Page 16 - The candela is the luminous intensity, in a given direction, of a source that emits monochromatic radiation of frequency 540 x 1012 hertz and that has a radiant intensity in that direction of (1/683) watt per steradian.
Page 17-2 - Science is built up with facts, as a house is with stones. But a collection of facts is no more a science than a heap of stones is a house.
Page 23 - Chirgwin, JM, Przybyla, AE, MacDonald, RJ, and Rutter, WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299.
Page 12-20 - Harlow E. and Lane D. 1988. Antibodies: A laboratory manual Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Page R-9 - Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase.
Page 27 - Yanisch-Perron, C., Vieira, J., and Messing, J. (1985). Improved M13 Phage Cloning Vectors and Host Strains: Nucleotide Sequences of the M13mpl8 and PUC19 Vectors.
Page 27 - Smith, DB, and Johnson, KS (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
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Sambrook J et al. 2000. Molecular Cloning: A Laboratory Manual ...
Molecular Cloning: A Laboratory Manual(Third Edition)
Scientific Commons: Molecular cloning : a laboratory manual / J ...
David W. Russell and Joseph Sambrook, author, Molecular Cloning
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