Recombinant DNAThis updated and revised second edition acts as an introduction to the conceps and techniques of recombinant DNA research and their results. The book features 14 new chapters and 11 rewritten chapters and incorperates research published throughout 1991. The coverage of recombinant DNA centres largely on key experiments, with sections focusing on new developments in cloning, mutagenisis, and genetic engineering, plus the contribution of recombinant DNA technology to our understanding of gene function, biological processes and human genetics. |
Contents
Replacement | 33 |
Phage DNA Can Insert into a Specific Site | 49 |
METHODS OF CREATING | 63 |
THE ISOLATION OF CLONED GENES | 99 |
THE COMPLEXITY OF THE GENOME | 135 |
TDNA Has Been Modified to Act as a Gene | 313 |
ONCOGENES AND ANTIONCOGENES | 335 |
ProtoOncogenes Encode Components | 389 |
RECOMBINANT DNA AND EVOLUTION | 433 |
The tat Gene Regulates Synthesis of HIV | 489 |
MAPPING AND CLONING HUMAN | 549 |
STUDYING WHOLE GENOMES | 583 |
Common terms and phrases
Acad activity allele amino acid sequence amino acids amplified analysis antibody antigen bacterial base pairs binding cancer carrying cDNA cell line cellular chain Chapter chromosome cloned cloned gene coding codon coli complementary complex containing culture cycle cyclin cytoplasm deletion detected disease DNA fragments DNA molecules DNA sequence domain Drosophila EcoRI elements embryo eukaryotic exons experiments Figure function gene expression genetic genome genomic DNA growth factor homologous hormone human hybrid infected inserted intron isolated ligated locus markers membrane mice molecular mouse mRNA mutagenesis mutations Natl neurons normal nucleotides oligonucleotide oncogene phage plants plasmid primers probe Proc produced promoter protein kinase reaction receptor recombinant DNA region replication repressor ribosomal RNA polymerase segments signal Southern blotting specific splicing strand structure studies subunit synthesis T-DNA target tissue transcription factors transfected transfer transformed transgenic transposon tumor vector viral virus viruses vitro wild-type yeast