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Molecular cloning:

a laboratory manual, Volume 1
Front Cover
5 Reviews
Cold Spring Harbor Laboratory, 1989 - Medical - 3 pages

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Review: Molecular Cloning: A Laboratory Manual

User Review  - Adrianna - Goodreads

Important book for someone in molecular biology field Read full review

Review: Molecular Cloning: A Laboratory Manual

User Review  - Sofia - Goodreads

This is the closest thing a molecular biologist has to Holy Scripture. There isn't a single molbio lab in the world that doesn't worship at the altar of Saint Sambrook, patron of DNA, RNA and protein. Goodreads needs a new rating system for this, because five stars isn't enough. Read full review

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Contents

Replication and Incompatibility 1
1-3
Constructing Expression Libraries in Plasmid and Bacteriophage
1-12
Oligonucleotidemediated Mutagenesis 15 51
1-15
Copyright

95 other sections not shown

Other editions - View all

Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual, Volume 3
Joseph Sambrook,David William Russell
Limited preview - 2001
Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual, Volume 3
Joseph Sambrook,E. F. Fritsch,Tom Maniatis
No preview available - 1989
Molecular Cloning: A Laboratory Manual
Molecular Cloning: A Laboratory Manual
Joseph Sambrook,Edward F. Fritsch,Thomas Maniatis
No preview available - 1989
View all »

Common terms and phrases

agar plate agarose gel aliquots ampicillin antibiotic bacteriophage particles bacteriophage T4 DNA BamHI buffer carry cDNA cells centrifugation at 12,000g Cl pH cleaved cloning coli containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic exonuclease extracts final concentration foreign DNA formamide gel electrophoresis gene genetic gradients Hindlll host hours at 37°C hybridization Incubate infected inserted ligation reaction linear lysogenic method microfuge tube minutes at 4°C minutes at room mixture mutations nitrocellulose nitrocellulose filter Nucleic Acids nucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified recA recombinant remove replication restriction enzymes RNAase room temperature segment of foreign sequences single-stranded DNA solution step sterile stored strains strand supernatant T4 DNA ligase teriophage transfer transformation vector DNA vitro volume

References to this book

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About the author (1989)

Sambrook was a senior staff scientist at Cold Spring Harbor Laboratory from 1969-1985. He is Professor of Research at the Peter MacCallum Cancer Centre, Melbourne.

Bibliographic information

QR code for Molecular cloning
TitleMolecular cloning: a laboratory manual, Volume 1
Molecular Cloning: A Laboratory Manual, E. F. Fritsch, ISBN 0879693096, 9780879693091
AuthorsJoseph Sambrook, E. F. Fritsch, Tom Maniatis
Photographs byE. F. Fritsch
Edition2, illustrated
PublisherCold Spring Harbor Laboratory, 1989
Original fromthe University of Michigan
Digitized23 Jul 2008
ISBN0879693096, 9780879693091
Length3 pages
Subjects ›  ›  › 

Eukaryotic cells
Medical / Genetics
Molecular cloning
Science / Life Sciences / Biology / Molecular Biology
Science / Life Sciences / Genetics & Genomics
  
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