PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 24
... automated liquid handler ) to function as the first automated system to perform this PCR method . The bed of this instrument , dubbed " Mr. Cycle ... Automated Instruments 25 manual operation , according to the requirements 24 PCR Automation.
... automated liquid handler ) to function as the first automated system to perform this PCR method . The bed of this instrument , dubbed " Mr. Cycle ... Automated Instruments 25 manual operation , according to the requirements 24 PCR Automation.
Page 55
... automation , 10 A schematic representation of the use of PCR in automating DNA sequencing is shown in Figure 6. A number of instruments for automated analysis of sequencing reaction are available , dsDNA SSDNA a b c def Figure 5 ...
... automation , 10 A schematic representation of the use of PCR in automating DNA sequencing is shown in Figure 6. A number of instruments for automated analysis of sequencing reaction are available , dsDNA SSDNA a b c def Figure 5 ...
Page 179
... automated fluorescent DNA sequencer that employs end - labeled fluorescent DNA primers.23,24 The manipulations for the automated DNA sequence analysis are essentially a scaling up of that for manual DNA sequencing . Generally , two ...
... automated fluorescent DNA sequencer that employs end - labeled fluorescent DNA primers.23,24 The manipulations for the automated DNA sequence analysis are essentially a scaling up of that for manual DNA sequencing . Generally , two ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube