PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 100
... cDNA as template . The PCR product thus generated should represent a portion of the cDNA coding for the protein of interest . Direct sequencing of this PCR product should yield enough sequence information to enable the synthesis of ...
... cDNA as template . The PCR product thus generated should represent a portion of the cDNA coding for the protein of interest . Direct sequencing of this PCR product should yield enough sequence information to enable the synthesis of ...
Page 174
... ( cDNA ) . The overall scheme is illustrated in Figure 3A and the experimental protocols described below ( Protocols A - C ) . First , cellular RNA is copied via reverse transcriptase to generate cDNA . Specific oligonucleotide primers ...
... ( cDNA ) . The overall scheme is illustrated in Figure 3A and the experimental protocols described below ( Protocols A - C ) . First , cellular RNA is copied via reverse transcriptase to generate cDNA . Specific oligonucleotide primers ...
Page 175
... cDNA 1st Strand CDNA Two Primers , PCR Dideoxynucleotide sequencing with end labeled primers BMW LN LN CLN Excess of Single Strands Single Strand Producing Reactions CTCAG Amplified CDNA TGTATTTGTACT ( G ) 175 Figure 3. A. Strategy for ...
... cDNA 1st Strand CDNA Two Primers , PCR Dideoxynucleotide sequencing with end labeled primers BMW LN LN CLN Excess of Single Strands Single Strand Producing Reactions CTCAG Amplified CDNA TGTATTTGTACT ( G ) 175 Figure 3. A. Strategy for ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
The Design and Optimization of the PCR | 7 |
PCR Amplification of Specific Sequences from | 9 |
Copyright | |
17 other sections not shown
Other editions - View all
PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequence dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target DNA target sequence temperature template Tris.HCl tube