Gene Cloning and ManipulationUpdated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams. |
Contents
Section 1 | 27 |
Section 2 | 30 |
Section 3 | 31 |
Section 4 | 47 |
Section 5 | 52 |
Section 6 | 55 |
Section 7 | 74 |
Section 8 | 79 |
Section 12 | 106 |
Section 13 | 109 |
Section 14 | 111 |
Section 15 | 113 |
Section 16 | 116 |
Section 17 | 143 |
Section 18 | 162 |
Section 19 | 180 |
Section 9 | 88 |
Section 10 | 98 |
Section 11 | 103 |
Section 20 | 182 |
Section 21 | 193 |
Section 22 | 243 |
Other editions - View all
Common terms and phrases
able acid activity addition allows amplified annealing approach appropriate bacterial bacteriophage binding called carried caused cDNA cells Chapter chromosome cloning codon coli colonies construct containing copy depends described detected direct DNA molecules DNA sequence efficiency encoding ends enzyme example exonuclease expression Figure followed fragments function fusion gene genetic genome give growth host hybrid identify important inactivated incorporated infection insert integration interest introduced lacZ lambda levels ligation methods methylated modified molecules mutation necessary nucleotide oligonucleotide organism origin packaging particular phage plant plasmid polymerase possible prepared presence primer probe problem promoter protein reaction recombination region removed replication reporter requires resistance restriction result screening selectable marker selection separate sequence shown single sometimes species strain strand suitable synthesis technique temperature template terminal transcription transfer transformation usually vector virus widely
Popular passages
Page 8 - The genus and species name of the host organism is identified by the first letter of the genus and the first two letters of the species to form a three-letter abbreviation in italics, eg Eco for E.