Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
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Page vii
... Insertional inactivation of a selectable marker 5.2.2 Insertional inactivation does not always involve antibiotic resistance 5.3 Introduction of phage DNA into bacterial cells 5.3.1 Phage infection is visualized as plaques on an agar ...
... Insertional inactivation of a selectable marker 5.2.2 Insertional inactivation does not always involve antibiotic resistance 5.3 Introduction of phage DNA into bacterial cells 5.3.1 Phage infection is visualized as plaques on an agar ...
Page 98
... INSERTIONAL INACTIVATION OF THE λ cI GENE Several types of λ cloning vector have unique restriction sites in the cl gene ( map position 38 on Figure 2.9 ) . Insertional inactivation of this gene causes a change in plaque morphology ...
... INSERTIONAL INACTIVATION OF THE λ cI GENE Several types of λ cloning vector have unique restriction sites in the cl gene ( map position 38 on Figure 2.9 ) . Insertional inactivation of this gene causes a change in plaque morphology ...
Page 104
... inactivate the amp gene , and insertion using any one of eight restriction endonucleases ( notably BamHI and HindIII ) inactivates tetracycline resistance . This great variety of restric- tion sites that can be used for insertional ...
... inactivate the amp gene , and insertion using any one of eight restriction endonucleases ( notably BamHI and HindIII ) inactivates tetracycline resistance . This great variety of restric- tion sites that can be used for insertional ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast