Molecular Cloning: A Laboratory Manual, Book 3 |
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Page 1674
... Natl . Acad . Sci . 76 : 3755 . Corsaro , C.M. and M.L. Pearson . 1981 . Enhancing the efficiency of DNA- mediated gene transfer in mammalian cells . Somatic Cell Mol . Genet . 7 : 603 . Cudd , A. and C. Nicolau . 1984. Entrap- ment of ...
... Natl . Acad . Sci . 76 : 3755 . Corsaro , C.M. and M.L. Pearson . 1981 . Enhancing the efficiency of DNA- mediated gene transfer in mammalian cells . Somatic Cell Mol . Genet . 7 : 603 . Cudd , A. and C. Nicolau . 1984. Entrap- ment of ...
Page 1676
... Natl . Acad . Sci . 79 : 5038 . Karin , M. , G. Cathala , and M.C. Nguyen- Huu . 1983. Expression and regulation of a human metallothionein gene car- ried on an autonomously replicating shuttle vector . Proc . Natl . Acad . Sci . 80 ...
... Natl . Acad . Sci . 79 : 5038 . Karin , M. , G. Cathala , and M.C. Nguyen- Huu . 1983. Expression and regulation of a human metallothionein gene car- ried on an autonomously replicating shuttle vector . Proc . Natl . Acad . Sci . 80 ...
Page 1677
... Natl . Acad . Sci . 83 : 2709 . Kühn , L.C. , A. McClelland , and F.H. Ruddle . 1984. Gene transfer , expres- sion , and molecular cloning of the human transferrin receptor gene . Cell 37:95 . Langner , K.-D. , U. Weyer , and W. Doer ...
... Natl . Acad . Sci . 83 : 2709 . Kühn , L.C. , A. McClelland , and F.H. Ruddle . 1984. Gene transfer , expres- sion , and molecular cloning of the human transferrin receptor gene . Cell 37:95 . Langner , K.-D. , U. Weyer , and W. Doer ...
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Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml