PCR Protocols: A Guide to Methods and ApplicationsMichael A. Innis The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to: * Optimize novel applications * Avoid many cumbersome molecular biological techniques * Set up the laboratory to avoid contamination |
From inside the book
Results 1-3 of 56
Page 468
... gel electrophoresis gel preparation , 215 parallel gel approach , 208 parallel procedure , 215-216 perpendicular gel approach , 208 perpendicular procedure , 216-217 preparation of PCR - amplified samples , 213-215 ras - specific ...
... gel electrophoresis gel preparation , 215 parallel gel approach , 208 parallel procedure , 215-216 perpendicular gel approach , 208 perpendicular procedure , 216-217 preparation of PCR - amplified samples , 213-215 ras - specific ...
Page 469
... gel electrophoresis , viral sequence products , 382-383 Ethidium bromide staining B - cell lymphoma translocation- specific fragments , 396 products of PCR with 7 - deaza - 2'- deoxyguanosine triphosphate , 56 Extension , primers , 7-8 ...
... gel electrophoresis , viral sequence products , 382-383 Ethidium bromide staining B - cell lymphoma translocation- specific fragments , 396 products of PCR with 7 - deaza - 2'- deoxyguanosine triphosphate , 56 Extension , primers , 7-8 ...
Page 477
... minigel electrophoresis , 375-376 genital human papillomavirus dot blot hybridization , 362-364 gel electrophoresis , 362 reagents and materials for analysis of , 361-362 restriction enzyme analysis , 364-365 Southern blot hybridization ...
... minigel electrophoresis , 375-376 genital human papillomavirus dot blot hybridization , 362-364 gel electrophoresis , 362 reagents and materials for analysis of , 361-362 restriction enzyme analysis , 364-365 Southern blot hybridization ...
Contents
Amplification of Genomic DNA | 13 |
Amplification of RNA | 21 |
Rapid Amplification of cDNA Ends | 28 |
Copyright | |
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Common terms and phrases
1990 by Academic Acad Academic Press agarose gel aliquots alleles amplification products amplified DNA analysis annealing Applications Copyright assay cDNA cells chromosome cloning concentration containing cycles denaturation detection digestion DNA fragments DNA sequences dNTP dot blot EDTA electrophoresis enzymatic amplification ethanol ethidium bromide extraction Faloona form reserved gene genetic genomic genomic DNA Guide to Methods H. A. Erlich Higuchi hybridization Incubate labeled lanes Literature Cited membrane Methods and Applications MgCl2 minutes molecular molecules mRNA mutations Natl Nucleic Acids Res nucleotide oligomer oligonucleotide probes PCR amplification PCR primers PCR product PCR Protocols Perkin-Elmer Cetus phage pmol polymerase chain reaction polymorphisms primer primer pair procedure purified quences reagents recombinant region restriction enzymes reverse transcriptase rights of reproduction rRNA Saiki Scharf single-stranded Sninsky solution Southern blot specific strand synthesis Taq DNA polymerase Taq polymerase temperature template tion tissue transcription Tris-HCl pH tube virus vitro