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1990 by Academic Academic Press agarose gel aliquots alleles amplification products amplified DNA analysis annealing Applications Copyright assay cDNA cells chromosome cloning concentration containing cycles denaturation detection digestion DNA fragments DNA sequences dNTP dot blot EDTA enzymatic amplification ethanol ethidium bromide extraction Faloona form reserved gene genetic genomic genomic DNA Guide to Methods H. A. Erlich Higuchi hybridization incubated labeled lanes Literature Cited membrane Methods and Applications MgCl2 minutes molecular molecules mRNA mutations Natl Nucleic Acids Res nucleotide oligomer oligonucleotide probes PCR amplification PCR primers PCR product PCR Protocols Perkin-Elmer Cetus phage pmol polymerase chain reaction polymorphisms prepared primer primer pair procedure purified quences reagents recombinant region restriction enzymes reverse transcriptase rights of reproduction rRNA Saiki Scharf single-stranded Sninsky solution Southern blot specific SSPE strand synthesis Taq DNA polymerase Taq polymerase temperature template tion tissue transcription Tris-HCl pH tube virus vitro
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