PCR Protocols: A Guide to Methods and Applications

Front Cover
Michael A. Innis
Academic Press, 1990 - Medical - 482 pages
The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to: * Optimize novel applications * Avoid many cumbersome molecular biological techniques * Set up the laboratory to avoid contamination

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Contents

Amplification of Genomic DNA
13
Amplification of RNA
21
Rapid Amplification of cDNA Ends
28
Copyright

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