The Condensed Protocols from Molecular Cloning: A Laboratory ManualThe Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning. |
Contents
77 | |
Ch 3 Working with Bacteriophage M13 Vectors | 133 |
Ch 4 Working with Highcapacity Vectors | 153 |
Ch 5 Gel Electrophoresis of DNA and Pulsedfield Agarose Gel Electrophoresis | 187 |
Ch 6 Preparation and Analysis of Eukaryotic Genomic DNA | 237 |
Ch 7 Extraction Purification and Analysis of mRNA from Eukaryotic Cells | 269 |
Ch 8 In Vitro Amplification of DNA by PCR | 309 |
Ch 9 Preparation of Radiolabeled DNA and RNA Probes | 357 |
Ch 15 Expression of Cloned Genes in E coli | 573 |
Ch 16 Introducing Cloned Genes into Cultured Mammalian Cells | 599 |
Ch17 Analysis of Gene Expression in Mammalian Cells | 621 |
Ch 18 Protein Interaction Technologies | 655 |
Appendices Index | 689 |
Appendix 1 Preparation of Reagents and Buffers Used in Molecular Cloning | 691 |
Appendix 2 Media | 717 |
Appendix 3 Commonly Used Techniques in Molecular Cloning | 727 |
Ch 10 Working with Synthetic Oligonucleotide Probes | 401 |
Ch 11 Preparation of cDNA Libraries and Gene Identification | 427 |
Ch 12 DNA Sequencing | 467 |
Ch 13 Mutagenesis | 505 |
Ch 14 Screening Expression Libraries | 543 |
Appendix 4 Cautions | 769 |
Index | 779 |
Back Cover | 801 |
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The Condensed Protocols from Molecular Cloning: A Laboratory Manual Joseph Sambrook,David William Russell No preview available - 2006 |
Common terms and phrases
acetate Adapted from Chapter Additional Additional Information Additional Items agar agarose gel aliquots allow amount amplification Appendix appropriate appropriate concentrations appropriate handling approximately bacterial bacteriophage buffer cDNA cells centrifugation chromatography cloned coli colonies column components of stock concentration containing culture denaturation digestion Dilute stock solutions Dissolve dNTPs EDTA ethanol ethidium expression extraction filters final fragments fresh Gels/Loading Buffers glass handling of materials heating hybridization Incubate labeled ligation MATERIALS CAUTION materials marked medium membrane METHOD mg/ml microfuge tube minutes minutes at 4°C minutes at room mixture NaCl nucleic acids oligonucleotide optional pellet Place plasmid DNA plates precipitation Prepare primer probe protein Protocol purified radiolabeled reaction reagents Reagents and Solutions remove restriction enzyme room temperature rotor sample seconds separate sequence sodium Sorvall staining Step sterile Store supernatant template transfer transformation Tris-Cl pH vector volume wash