Gene Cloning: An Introduction |
From inside the book
Results 1-3 of 8
Page 289
... BRCA1 Figure 13.10 Mapping the breast cancer gene . Initially the gene was mapped to a 20 - Mb segment of chromosome 17 ( highlighted region in the left drawing ) . Additional map- ping experiments narrowed this down to a 600 - kb ...
... BRCA1 Figure 13.10 Mapping the breast cancer gene . Initially the gene was mapped to a 20 - Mb segment of chromosome 17 ( highlighted region in the left drawing ) . Additional map- ping experiments narrowed this down to a 600 - kb ...
Page 290
... BRCA1 from among these expressed sequences : 1. Hybridization probes specific for individual expressed sequences were used to examine RNA samples from different tissues . A candidate for BRCA1 would be expected to hybridize to RNA ...
... BRCA1 from among these expressed sequences : 1. Hybridization probes specific for individual expressed sequences were used to examine RNA samples from different tissues . A candidate for BRCA1 would be expected to hybridize to RNA ...
Page 291
... BRCA1 . ( c ) The next set of questions The identification of any gene responsible for an inherited disease is greeted by the scientific ( and sometimes popular ) press as a cause for celebration . In reality it is just the first step ...
... BRCA1 . ( c ) The next set of questions The identification of any gene responsible for an inherited disease is greeted by the scientific ( and sometimes popular ) press as a cause for celebration . In reality it is just the first step ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
13 other sections not shown
Other editions - View all
Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast