Gene Cloning: An Introduction |
From inside the book
Results 1-3 of 33
Page 195
... DNA so the newly synthesized strands dissociate from the templates . In addition , the elec- trophoresis is carried out at a high voltage , so the gel heats up to 60 ° C and above , making sure the strands do not ... DNA sequencing 195.
... DNA so the newly synthesized strands dissociate from the templates . In addition , the elec- trophoresis is carried out at a high voltage , so the gel heats up to 60 ° C and above , making sure the strands do not ... DNA sequencing 195.
Page 199
An Introduction Terence A. Brown. individual DNA sequences will themselves overlap . The overlaps can be located , either by eye or using a computer , and the master sequence gradually built up . There are several ways ... DNA sequencing 199.
An Introduction Terence A. Brown. individual DNA sequences will themselves overlap . The overlaps can be located , either by eye or using a computer , and the master sequence gradually built up . There are several ways ... DNA sequencing 199.
Page 205
... sequencing DNA . Proceedings of the National Academy of Sciences , USA , 74 , 560-4 . - Brown , T. A. ( 1994 ) DNA Sequencing : The Basics . Oxford University Press , Oxford everything you need to know about DNA sequencing . Johnston ...
... sequencing DNA . Proceedings of the National Academy of Sciences , USA , 74 , 560-4 . - Brown , T. A. ( 1994 ) DNA Sequencing : The Basics . Oxford University Press , Oxford everything you need to know about DNA sequencing . Johnston ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
13 other sections not shown
Other editions - View all
Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast