Gene Cloning: An Introduction |
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Page 10
... amplified . 1.6 WHY THE POLYMERASE CHAIN REACTION IS ALSO IMPORTANT Until the late 1980s cloning was the only means ... amplified DNA fragment ( Figure 1.4 ) . The experiment is designed so that the seg- ment of DNA that is amplified is ...
... amplified . 1.6 WHY THE POLYMERASE CHAIN REACTION IS ALSO IMPORTANT Until the late 1980s cloning was the only means ... amplified DNA fragment ( Figure 1.4 ) . The experiment is designed so that the seg- ment of DNA that is amplified is ...
Page 231
... amplified . Alternatively the PCR product can be ligated into a plas- mid or bacteriophage vector , cloned in the normal way , and exam- ined by standard techniques such as DNA sequencing . 11.2 PCR IN MORE DETAIL Although PCR ...
... amplified . Alternatively the PCR product can be ligated into a plas- mid or bacteriophage vector , cloned in the normal way , and exam- ined by standard techniques such as DNA sequencing . 11.2 PCR IN MORE DETAIL Although PCR ...
Page 242
... amplified fragment that has an error at a particular nucleotide position there will be many molecules with the correct sequence . In this context the error rate is indeed insignificant . This is not the case if the PCR products are ...
... amplified fragment that has an error at a particular nucleotide position there will be many molecules with the correct sequence . In this context the error rate is indeed insignificant . This is not the case if the PCR products are ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast