Gene Cloning: An Introduction |
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Page 112
... cloned gene is potentially harmful should an accident occur . ( b ) pUC8- a Lac selection plasmid ( Figure 6.3 ( b ) ) This vector was mentioned in Chapter 5 when identification of recombinants by insertional inactivation of the B ...
... cloned gene is potentially harmful should an accident occur . ( b ) pUC8- a Lac selection plasmid ( Figure 6.3 ( b ) ) This vector was mentioned in Chapter 5 when identification of recombinants by insertional inactivation of the B ...
Page 183
... gene of interest . In many cases , the next stage in the research project will be to purify the recombi- nant DNA molecule and obtain from it as much information as ... gene and genome structure How to study the location of a cloned gene.
... gene of interest . In many cases , the next stage in the research project will be to purify the recombi- nant DNA molecule and obtain from it as much information as ... gene and genome structure How to study the location of a cloned gene.
Page 217
... cloned gene is regulated ( Figure 10.11 ) . For instance , deletion of a sequence that represses expression of a gene should result in that gene being expressed at a higher level . Similarly , tis- sue - specific control sequences can ...
... cloned gene is regulated ( Figure 10.11 ) . For instance , deletion of a sequence that represses expression of a gene should result in that gene being expressed at a higher level . Similarly , tis- sue - specific control sequences can ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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Common terms and phrases
agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast