Gene Cloning: An Introduction |
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Page 35
... nucleic acids . When CTAB is added to a plant cell extract the nucleic acid - CTAB complex precipitates , leaving carbohydrate , protein and other contaminants in the supernatant ... Nucleic acid- CTAB Preparation of total cell DNA 35.
... nucleic acids . When CTAB is added to a plant cell extract the nucleic acid - CTAB complex precipitates , leaving carbohydrate , protein and other contaminants in the supernatant ... Nucleic acid- CTAB Preparation of total cell DNA 35.
Page 208
... DNA mole- cule for observation with the electron microscope . 10.1.1 Electron microscopy of nucleic acid molecules Electron microscopy can be used to visualize nucleic acid mole- cules , so long as the polynucleotides are first treated ...
... DNA mole- cule for observation with the electron microscope . 10.1.1 Electron microscopy of nucleic acid molecules Electron microscopy can be used to visualize nucleic acid mole- cules , so long as the polynucleotides are first treated ...
Page 316
... nucleic acid molecule . Episome A plasmid capable of integration into the host cell's chromosome . Ethanol precipitation Precipitation of nucleic acid molecules by ethanol plus salt , used primarily as a means of concentrating DNA ...
... nucleic acid molecule . Episome A plasmid capable of integration into the host cell's chromosome . Ethanol precipitation Precipitation of nucleic acid molecules by ethanol plus salt , used primarily as a means of concentrating DNA ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast