Gene Cloning: An Introduction |
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Page 19
An Introduction Terence A. Brown. Phage particle DNA 1 The phage attaches to the bacterium and injects its DNA Phage ... particles are assembled and released Cell lysis New phage particles จ The general pattern of infection , which is the ...
An Introduction Terence A. Brown. Phage particle DNA 1 The phage attaches to the bacterium and injects its DNA Phage ... particles are assembled and released Cell lysis New phage particles จ The general pattern of infection , which is the ...
Page 48
... phage from an infected culture The remains of lysed bacterial cells , along with any intact cells that are inadvertently left over , can be removed from an infected culture by centrifugation , leaving the phage particles in suspension ...
... phage from an infected culture The remains of lysed bacterial cells , along with any intact cells that are inadvertently left over , can be removed from an infected culture by centrifugation , leaving the phage particles in suspension ...
Page 49
An Introduction Terence A. Brown. 1.35 1.40 1.45 λ phage particles 1.50 1.55 1.60 CsCl density ( g / cm3 ) Figure 3.20 Purification of λ phage particles by CSCI density gradient centrifugation . from which the DNA can be extracted by ...
An Introduction Terence A. Brown. 1.35 1.40 1.45 λ phage particles 1.50 1.55 1.60 CsCl density ( g / cm3 ) Figure 3.20 Purification of λ phage particles by CSCI density gradient centrifugation . from which the DNA can be extracted by ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast