Gene Cloning: An Introduction |
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Page 39
... polynucleotide strands are intact , hence the more technical name of covalently closed - circular ( ccc ) DNA . If one of the polynucleotide strands is broken , then the double helix will revert to its normal , relaxed state , and the ...
... polynucleotide strands are intact , hence the more technical name of covalently closed - circular ( ccc ) DNA . If one of the polynucleotide strands is broken , then the double helix will revert to its normal , relaxed state , and the ...
Page 59
... Polynucleotide kinase ( from E. coli infected with T4 phage ) which has the reverse effect to alkaline phosphatase , adding phosphate groups on to free 5 ' - termini ( Figure 4.6 ( b ) ) . 3. Terminal deoxynucleotidyl transferase ( from ...
... Polynucleotide kinase ( from E. coli infected with T4 phage ) which has the reverse effect to alkaline phosphatase , adding phosphate groups on to free 5 ' - termini ( Figure 4.6 ( b ) ) . 3. Terminal deoxynucleotidyl transferase ( from ...
Page 324
... polynucleotide . Sticky end An end of a double - stranded DNA molecule where there is a single - stranded extension ... polynucleotide ( or region of a polynucleotide ) able to direct synthesis of a complementary polynucleotide ...
... polynucleotide . Sticky end An end of a double - stranded DNA molecule where there is a single - stranded extension ... polynucleotide ( or region of a polynucleotide ) able to direct synthesis of a complementary polynucleotide ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast