Gene Cloning: An Introduction |
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Page 52
... purified enzymes . Within the cell these enzymes participate in essential processes such as DNA replication and transcription , breakdown of unwanted or foreign DNA ( for example , invading virus DNA ) , repair of mutated DNA , and ...
... purified enzymes . Within the cell these enzymes participate in essential processes such as DNA replication and transcription , breakdown of unwanted or foreign DNA ( for example , invading virus DNA ) , repair of mutated DNA , and ...
Page 180
... Purified antibod- ies can be obtained from a small vol- ume of blood taken from a rabbit injected with the foreign protein . ( a ) Antibodies bind to foreign molecules ( b ) Antibody purification Rabbit injected with foreign protein ...
... Purified antibod- ies can be obtained from a small vol- ume of blood taken from a rabbit injected with the foreign protein . ( a ) Antibodies bind to foreign molecules ( b ) Antibody purification Rabbit injected with foreign protein ...
Page 238
... purified . A clever way of doing this is by attaching small magnetic beads to one of the primers . After the PCR , single - stranded DNA is obtained by separating the ' mag- netic ' strand from the ordinary strand ( Figure 11.10 ) . A ...
... purified . A clever way of doing this is by attaching small magnetic beads to one of the primers . After the PCR , single - stranded DNA is obtained by separating the ' mag- netic ' strand from the ordinary strand ( Figure 11.10 ) . A ...
Contents
Further reading | 12 |
Purification of DNA from living cells | 27 |
Further reading | 50 |
Copyright | |
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Common terms and phrases
able acid addition amount amplified analysis animal attached bacterial bacteriophage BamHI bands Biotechnology carried cDNA cell Chapter chromosome cloned gene cloning vectors coded coli colonies complete construction containing copy correct culture deletion desired detected developed direct disease DNA fragment DNA molecule EcoRI electrophoresis ends engineering enzyme example experiment expression Figure gene cloning genetic genome glycosylation host human hybridization identified important individual infection inserted involved labelled ligated marker means medium method molecular mRNA mutation natural needed normal nucleotide obtained occur organisms origin phage plant plaques plasmid polymerase position possible preparation present primers probe problem promoter protein purified reaction recombinant DNA molecule region removed replication resistance result segment selection separated sequence single single-stranded specific strand structure synthesis T-DNA techniques tion transcription transfer transformed translation types usually virus yeast