Gene Cloning: An Introduction |
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Page 195
... strands all ending in dideoxyATP , one of strands ending in dideoxyTTP , etc. The next step is to separate the components of each family so the lengths of each strand can be determined . This can be achieved by gel electrophoresis ...
... strands all ending in dideoxyATP , one of strands ending in dideoxyTTP , etc. The next step is to separate the components of each family so the lengths of each strand can be determined . This can be achieved by gel electrophoresis ...
Page 196
... strand synthesis in the presence of one of the four dideoxyNTPs . The sequence is read by identifying the track that each fragment lies in , starting with the one that has moved the furthest , and gradually progressing up through the ...
... strand synthesis in the presence of one of the four dideoxyNTPs . The sequence is read by identifying the track that each fragment lies in , starting with the one that has moved the furthest , and gradually progressing up through the ...
Page 238
... strand from the ordinary strand ( Figure 11.10 ) . A similar method makes use of a biotin - labelled primer , with the single strands separated by binding to avidin , a protein that has a high affinity for biotin ( p . 172 ) . Once ...
... strand from the ordinary strand ( Figure 11.10 ) . A similar method makes use of a biotin - labelled primer , with the single strands separated by binding to avidin , a protein that has a high affinity for biotin ( p . 172 ) . Once ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast