Gene Cloning: An Introduction |
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Page 58
... Template Primer 3 ' 5 ' 3 ' 5 ' Newly synthesized strand ( b ) DNA polymerase I -A - T - G - C - A - A - T - G - C - A - T- -T - A - C - G G - T - A- A nick ( c ) The Klenow fragment -A - T - G - C - A - A - T - G - C - A - T- -T - A ...
... Template Primer 3 ' 5 ' 3 ' 5 ' Newly synthesized strand ( b ) DNA polymerase I -A - T - G - C - A - A - T - G - C - A - T- -T - A - C - G G - T - A- A nick ( c ) The Klenow fragment -A - T - G - C - A - A - T - G - C - A - T- -T - A ...
Page 231
... template molecule . Each primer must of course be complementary ( not identical ) to its template strand in order for Size markers 1 2 3 4 Figure 11.3 The results of PCRs with well - designed and poorly- designed primers . Lane 1 shows ...
... template molecule . Each primer must of course be complementary ( not identical ) to its template strand in order for Size markers 1 2 3 4 Figure 11.3 The results of PCRs with well - designed and poorly- designed primers . Lane 1 shows ...
Page 235
... template molecule . The ideal annealing temperature must be low enough to enable hybridization between primer and template , but high enough to prevent mismatched hybrids from forming ( Figure 11.7 ( c ) ) . This temperature can be ...
... template molecule . The ideal annealing temperature must be low enough to enable hybridization between primer and template , but high enough to prevent mismatched hybrids from forming ( Figure 11.7 ( c ) ) . This temperature can be ...
Contents
Further reading | 12 |
Manipulation of purified | 56 |
Introduction of DNA into living cells | 88 |
Copyright | |
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agarose gel amino acid amplified ampR analysis animal antibody antisense autoradiograph bacterial bacteriophage bacterium BamHI base-pairing Biotechnology BRCA1 carried cDNA cell extract cerevisiae cloned gene cloning experiment cloning vectors coded codons coli colonies containing control sequence cosmid culture deletion disease DNA fragment DNA sequencing double-stranded E.coli EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression genetic engineering Genetic fingerprinting genomic library host cell human hybridization probing identified infection inserted introns labelled lacZ LEU2 ligated medium method molecular mRNA mutation normal nucleic acid nucleotide nucleotide sequence obtained oligonucleotide organisms PCR product phage particles plaques plasmid polylinker polynucleotide primers problem promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication resistance restriction endonuclease restriction fragment restriction sites result RFLP segment selectable marker single-stranded DNA sticky ends strand synthesis T-DNA techniques template Ti plasmid tion transcription translation trpA types virus viruses vitro yeast