Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 5-36
... exonuclease , and a 3 ' 5 ' exonuclease ( Kelley and Stump 1979 ) and that has an inherent RNAase H activity . The RNAase H activity is essential for cell viability in E. coli but has not been used in molecular cloning . → 1. Labeling ...
... exonuclease , and a 3 ' 5 ' exonuclease ( Kelley and Stump 1979 ) and that has an inherent RNAase H activity . The RNAase H activity is essential for cell viability in E. coli but has not been used in molecular cloning . → 1. Labeling ...
Page 5-84
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Exonuclease III ( E. coli ) USES Exonuclease III catalyzes the stepwise removal of 5 ′ mononucleotides from the 3 ' - hydroxyl termini of double - stranded DNA ( Weiss ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Exonuclease III ( E. coli ) USES Exonuclease III catalyzes the stepwise removal of 5 ′ mononucleotides from the 3 ' - hydroxyl termini of double - stranded DNA ( Weiss ...
Page 5-86
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Bacteriophage Exonuclease ( Bacteriophage A - infected E. coli ) USES Bacteriophage exonuclease catalyzes the processive , stepwise release of 5 ' mononucleotides from ...
A Laboratory Manual Joseph Sambrook, E. F. Fritsch, Tom Maniatis. Bacteriophage Exonuclease ( Bacteriophage A - infected E. coli ) USES Bacteriophage exonuclease catalyzes the processive , stepwise release of 5 ' mononucleotides from ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml