Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 87
Page 1-3
... Replication and Incompatibility Replication of plasmid DNA is carried out by subsets of enzymes used to duplicate the bacterial chromosome . However , different plasmids use differ- ent subsets and replicate to different extents in ...
... Replication and Incompatibility Replication of plasmid DNA is carried out by subsets of enzymes used to duplicate the bacterial chromosome . However , different plasmids use differ- ent subsets and replicate to different extents in ...
Page 3-19
... replication and two copies of cos oriented in tandem . This arrangement of cos sequences increases the efficiency ... replication , both derived from SV2 - gpt ( Mulligan and Berg 1980 ) . pCV107 contains the mouse dihydrofolate ...
... replication and two copies of cos oriented in tandem . This arrangement of cos sequences increases the efficiency ... replication , both derived from SV2 - gpt ( Mulligan and Berg 1980 ) . pCV107 contains the mouse dihydrofolate ...
Page 4-17
... REPLICATION DERIVED FROM A FILAMENTOUS BACTERIOPHAGE Several vectors have been developed that combine desirable features of both plasmids and filamentous bacteriophages . In their simplest form , these vectors are plasmids with a ColE1 ...
... REPLICATION DERIVED FROM A FILAMENTOUS BACTERIOPHAGE Several vectors have been developed that combine desirable features of both plasmids and filamentous bacteriophages . In their simplest form , these vectors are plasmids with a ColE1 ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml