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Molecular cloning:

a laboratory manual, Volume 1
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Cold Spring Harbor Laboratory, Sep 1, 1989 - Medical - 1659 pages

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Contents

Plasmid Vectors
1-2
DEVELOPMENT OF PLASMID CLONING VECTORS 1
1-8
Constructing Expression Libraries in Plasmid and Bacteriophage
1-12
Copyright

85 other sections not shown

Common terms and phrases

agarose gel aliquots bacteria bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer carrying cDNA cells centrifugation at 12,000g Cl pH cleavage cleaved cloning cohesive termini coli DNA polymerase containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA efficiently Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts final concentration foreign DNA gel electrophoresis gene genetic Hindlll hours at 37°C hybridization Incubate infected inserted intergenic region ligation reaction method methylation microfuge tube minutes at 4°C minutes at room mutations Natl nitrocellulose nitrocellulose filter Nucleic Acids Nucleic Acids Res nucleotides origin of replication packaging pellet phagemid plaques plasmid plasmid DNA polycloning prepared probes protein purified recA recombinant remove restriction enzymes room temperature segment of foreign single-stranded DNA solution sterile stored strains strand supernatant T4 DNA ligase template teriophage Transfer vector DNA vitro volume xg/ml

About the author (1989)

Sambrook was a senior staff scientist at Cold Spring Harbor Laboratory from 1969-1985. He is Professor of Research at the Peter MacCallum Cancer Centre, Melbourne.

Bibliographic information

QR code for Molecular cloning
TitleMolecular cloning: a laboratory manual, Volume 1
Molecular Cloning: A Laboratory Manual, E. F. Fritsch, ISBN 0879693096, 9780879693091
AuthorsJoseph Sambrook, E. F. Fritsch, Tom Maniatis
Photographs byE. F. Fritsch
Edition2, illustrated
PublisherCold Spring Harbor Laboratory, 1989
Original fromthe University of California
Digitized27 Jun 2009
ISBN0879693096, 9780879693091
Length1659 pages
Subjects ›  › 

Eukaryotic cells
Medical / Genetics
Molecular cloning
Science / Life Sciences / Genetics & Genomics
Science / Life Sciences / Molecular Biology
  
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