Polymerase Chain Reaction

Acad alleles amplified DNA assay B-thalassemia base cDNA Cetus chain reaction PCR chromosome clones codon containing cosmid cycles D.H. Gelfand deletions denaturing gradient gel detection diagnosis direct sequencing disease DNA fragments DNA sequence DNA synthesis dNTP Enzymatic amplification enzyme error exon Faloona Fidelity of DNA G.T. Horn gel electrophoresis genetic genomic DNA H.A. Erlich haplotype HPRT HTLV-I hybridization identification infected K.B. Mullis Kunkel Kwok lane Lerman ligation locus method mismatch molecular molecules mRNA Natl normal Nucleic Acids Res nucleotide oligonucleotide probes patients PCR amplification PCR primers PCR product Poiesz polymerase chain reaction polymorphism Proc protein quence recombinant region retroviral retroviruses RNase-A Saiki samples Scharf Science 239 screening sequence analysis Sfil sickle cell anemia single single-base Sninsky Southern blot specific ẞ-globin strand subcloning Taq DNA polymerase Taq polymerase target technique temperature Thermus aquaticus tion vector viral virus vitro