Physical Principles and Techniques of Protein ChemistrySydney J. Leach Physical Principles and Techniques of Protein Chemistry Part C ... |
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Page 20
... concentrations are kept very low . 1. Protein Concentration This short discussion is an aside to the main theme of this chapter . Accurate values for the protein concentration , however , are of critical importance in order to derive ...
... concentrations are kept very low . 1. Protein Concentration This short discussion is an aside to the main theme of this chapter . Accurate values for the protein concentration , however , are of critical importance in order to derive ...
Page 51
... protein , when a system proceeds to osmotic equilibrium from an initial isomolal condition . The introduction of protein ... concentration of component 3 is decreased , the net amount of diffusible components transported in either direction ...
... protein , when a system proceeds to osmotic equilibrium from an initial isomolal condition . The introduction of protein ... concentration of component 3 is decreased , the net amount of diffusible components transported in either direction ...
Page 103
Sydney J. Leach. ble components , it is clear that as the concentration of protein approaches zero , differences in the diffusible ion concentration and in the activity coefficients on the two sides of the membrane become negligible ...
Sydney J. Leach. ble components , it is clear that as the concentration of protein approaches zero , differences in the diffusible ion concentration and in the activity coefficients on the two sides of the membrane become negligible ...
Contents
Direct Volume Change | 22 |
Density | 32 |
Derivation of the Partial Specific Volume from | 62 |
Copyright | |
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a-helix absorption acid Acta angle axial ratio B-structure band Biochem Biochemistry Biol buffer c₂ calculated CD spectra Chem chromophore circular dichroism coefficient complex conformation constant Cotton effects cross-section curves denaturation determined dialyzate diffusible components dilute electron density ellipsoids ellipticity enzyme equation equilibrium exciton factor function gm/ml grams Guinier helical helix hemocyanin hemoglobin intensity interaction investigated Kratky lysozyme macromolecule magnetic measurements membrane method mg/ml molal molecular weight molecule myoglobin negative nondiffusible obtained optical activity osmometers osmotic pressure parameter partial specific volume particles peptide Phys Pilz plot polymer polypeptides Porod primary beam procedure protein solution pulse radii radius of gyration residues rotational strength sample Schellman Section shape small-angle scattering small-angle X-ray scattering solvent solvent medium spectrum structure studies subsidiary maxima subunits T₁ temperature theoretical scattering curves Tinoco tion transition v₂ wavelength zero