GB/T 40175.2-2021 Translated English of Chinese Standard (GBT40175.2-2021): Textiles - Methods of biochemical analysis - Part 2: Pyrethroid pesticides(enzyme-linked immunosorbent assay) [Buy--download True-PDF in 3-second: https://play.google.com/books > Sign in > This BOOK > "3-dots" > Export > Save as "*.pdf"]This document specifies the enzyme-linked immunosorbent assay method for the detection of residues of seven pyrethroid pesticides, including fenpropathrin, deltamethrin, cypermethrin, beta-cypermethrin, cyfluthrin, RU 38702, and cyphenothrin, in textiles. This document applies to all kinds of textiles. |
Contents
| 3 | |
Scope | 4 |
Reagents and materials | 5 |
Equipment and utensils | 6 |
Calculation and expression of results | 7 |
Detection limit | 9 |
Appendix A Informative Example of pyrethroid pesticide enzymelinked immunosorbent assay kit | 10 |
References | 11 |
Common terms and phrases
30 minutes absorbent paper Add 50 µL antibody working solution aqueous solution average absorbance value Beta-cypermethrin biochemical analysis Calculate CAS number citric acid Color-developing agent concentrated washing solution contains 13.6 g content of pyrethroid Cyfluthrin Cypermethrin Cyphenothrin Deltamethrin determination of pyrethroid discard the liquid disodium hydrogen phosphate document dry on absorbent dry the remaining enzyme-labeled secondary antibody enzyme-linked immunosorbent assay Fenpropathrin standard Fill each hole g of citric g of potassium g of sodium GB/T hole with washing methanol Methanol-phosphate buffer solution micrograms microplate reader microtiter plate 5.1a Nanjing Customs oven pesticide enzyme-linked immunosorbent Petroleum ether Products Testing Center Pyrethroid antibody Pyrethroid pesticides enzyme-linked reagents relative absorbance value repeat the washing sample solution series standard shake gently sodium acetate sodium chloride solution that contains standard solution standard working curve standard working solution Stop solution Substrate solution temperature control accuracy Textile ultrasonic bath volume ratio washing liquid 5.2 µg/kg µg/L