Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-57
... adjust the pH of each of the solutions to 7.0 ( use pH paper to check the pH ) . Dilute an aliquot of the neutralized dNTP appropriately , and read the optical density at the wavelengths given in the table below . Calculate the actual ...
... adjust the pH of each of the solutions to 7.0 ( use pH paper to check the pH ) . Dilute an aliquot of the neutralized dNTP appropriately , and read the optical density at the wavelengths given in the table below . Calculate the actual ...
Page 13-39
... adjust the buffer and add the second enzyme . Any DNA that escapes cleavage by the second enzyme will be digested in both directions by exonuclease III and will therefore be unlikely to generate viable clones . To adjust the restriction ...
... adjust the buffer and add the second enzyme . Any DNA that escapes cleavage by the second enzyme will be digested in both directions by exonuclease III and will therefore be unlikely to generate viable clones . To adjust the restriction ...
Page 13-77
... Adjust ratio of ddNTP to dNTP Adjust ratio of ddNTP to dNTP Increase the amount of template Use a primer more distant from the target site Check that specific activity of [ 35S ] dATP is 600 Ci / mmole Add 1 μl of [ 35S ] dATP to the ...
... Adjust ratio of ddNTP to dNTP Adjust ratio of ddNTP to dNTP Increase the amount of template Use a primer more distant from the target site Check that specific activity of [ 35S ] dATP is 600 Ci / mmole Add 1 μl of [ 35S ] dATP to the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml