Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 85
Page 8-42
... Chapter 2 ) , and ( 2 ) the SacI and XbaI sites were eliminated from the vector arms so that the XbaI site in the polycloning site is unique ( see map of Agt20 in Chapter 2 , page 2.46 ) . These manipulations resulted in deletion of ...
... Chapter 2 ) , and ( 2 ) the SacI and XbaI sites were eliminated from the vector arms so that the XbaI site in the polycloning site is unique ( see map of Agt20 in Chapter 2 , page 2.46 ) . These manipulations resulted in deletion of ...
Page 8-74
... Chapter 2 , page 2.104 ) . Because the size of the final cDNA library depends on the efficiency with which recombinant bacteriophage A genomes are packaged λ into infectious bacteriophage particles , it is essential to use packaging ...
... Chapter 2 , page 2.104 ) . Because the size of the final cDNA library depends on the efficiency with which recombinant bacteriophage A genomes are packaged λ into infectious bacteriophage particles , it is essential to use packaging ...
Page 15-72
... Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and ... Chapter 1. Incubate the bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the ...
... Chapter 11. However , because putative positive colonies almost certainly contain a mixture of wild - type and ... Chapter 1. Incubate the bacterial plates for a few hours at 37 ° C to allow the colonies to regrow , and then store the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml