Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-16
... EDTA ( pH 8.0 ) in the extraction buffer . However , the density of this solution almost equals that of phenol , which makes separation of the phases in step 4 difficult . In our hands , 0.1 M EDTA ( pH 8.0 ) is equally effective in ...
... EDTA ( pH 8.0 ) in the extraction buffer . However , the density of this solution almost equals that of phenol , which makes separation of the phases in step 4 difficult . In our hands , 0.1 M EDTA ( pH 8.0 ) is equally effective in ...
Page 11-35
... EDTA - Tris ( pH 6.0 ) Add 0.1 mole of EDTA ( free acid m.w. = 292.2 ) to 60 ml of H2O . While stirring the solution , slowly add Tris base ( powder ) until the pH of the solution reaches 6.0 . By this stage , the concentration of Tris ...
... EDTA - Tris ( pH 6.0 ) Add 0.1 mole of EDTA ( free acid m.w. = 292.2 ) to 60 ml of H2O . While stirring the solution , slowly add Tris base ( powder ) until the pH of the solution reaches 6.0 . By this stage , the concentration of Tris ...
Page 13-84
... EDTA ( pH 8.0 ) 100 μg / ml yeast tRNA 5 M NaCl NaOH / EDTA solution 1 M Piperidine in H2O 1 M Piperidine formate ( pH 2.0 ) Salmon sperm DNA or calf thymus DNA Sequencing gel - loading buffer 1.2 N NaOH 1 mM EDTA This solution should ...
... EDTA ( pH 8.0 ) 100 μg / ml yeast tRNA 5 M NaCl NaOH / EDTA solution 1 M Piperidine in H2O 1 M Piperidine formate ( pH 2.0 ) Salmon sperm DNA or calf thymus DNA Sequencing gel - loading buffer 1.2 N NaOH 1 mM EDTA This solution should ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml