Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-78
... Figure 13.2 , page 13.12 ) . The sequence of nucleotides lying within 250 nucleotides of the labeled terminus can ... Figure 13.10 . A useful set of plasmid vectors that facilitate asymmetric labeling of double - stranded fragments of ...
... Figure 13.2 , page 13.12 ) . The sequence of nucleotides lying within 250 nucleotides of the labeled terminus can ... Figure 13.10 . A useful set of plasmid vectors that facilitate asymmetric labeling of double - stranded fragments of ...
Page 14-12
... Figure 14.2 , genomic DNA is digested to completion with a restriction enzyme that has no sites of cleavage within the target sequence . The fragment carrying the target sequence should be no larger than 2-3 kb in length . The DNA is ...
... Figure 14.2 , genomic DNA is digested to completion with a restriction enzyme that has no sites of cleavage within the target sequence . The fragment carrying the target sequence should be no larger than 2-3 kb in length . The DNA is ...
Page 15-57
... Figure 15.7 ) , two oligonucleotides - the phosphorylated mutagenic oligonucleotide and a universal sequencing primer ( which need not be phosphorylated ) -are mixed in a 10- to 50 - fold molar excess with the single - stranded template ...
... Figure 15.7 ) , two oligonucleotides - the phosphorylated mutagenic oligonucleotide and a universal sequencing primer ( which need not be phosphorylated ) -are mixed in a 10- to 50 - fold molar excess with the single - stranded template ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml