Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 9-17
... incubate for 1 hour at 37 ° C , and then proceed to step 2 . Caution : It has been well - documented that human blood , blood products , and tissues may contain occult infectious materials such as hepatitis B virus and human ...
... incubate for 1 hour at 37 ° C , and then proceed to step 2 . Caution : It has been well - documented that human blood , blood products , and tissues may contain occult infectious materials such as hepatitis B virus and human ...
Page 13-40
... incubate the microfuge tubes or microtiter plate for 30 minutes at 30 ° C . Add 1 μl of S1 stop mixture to each of the microfuge tubes or wells , and incubate for 10 minutes at 70 ° C . This inactivates nuclease S1 and any residual ...
... incubate the microfuge tubes or microtiter plate for 30 minutes at 30 ° C . Add 1 μl of S1 stop mixture to each of the microfuge tubes or wells , and incubate for 10 minutes at 70 ° C . This inactivates nuclease S1 and any residual ...
Page 13-96
... Incubate 7 minutes at 37 ° C . Chill to 0 ° C . Add 150 μl 1 M piperidine ( at 0 ° C ) . Store on ice until all reactions are ready for incubation . Incubate 30 minutes at 90 ° C . Add 1.2 ml 1 - butanol . Vortex for 30 seconds . A > C ...
... Incubate 7 minutes at 37 ° C . Chill to 0 ° C . Add 150 μl 1 M piperidine ( at 0 ° C ) . Store on ice until all reactions are ready for incubation . Incubate 30 minutes at 90 ° C . Add 1.2 ml 1 - butanol . Vortex for 30 seconds . A > C ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector