Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 70
Page 11-40
... Klenow fragment of E. coli DNA polymer- ase I to synthesize a strand of DNA complementary to the synthetic oligonu- cleotide ( Studencki and Wallace 1984 ; Ullrich et al . 1984b ) ( Figure 11.5 ) . A short primer is hybridized to an ...
... Klenow fragment of E. coli DNA polymer- ase I to synthesize a strand of DNA complementary to the synthetic oligonu- cleotide ( Studencki and Wallace 1984 ; Ullrich et al . 1984b ) ( Figure 11.5 ) . A short primer is hybridized to an ...
Page 13-8
... Klenow enzyme than with Sequenases , perhaps because they decrease still further the already low processivity of the enzyme . In summary , the Klenow fragment of E. coli DNA polymerase I is the enzyme of choice for determining the ...
... Klenow enzyme than with Sequenases , perhaps because they decrease still further the already low processivity of the enzyme . In summary , the Klenow fragment of E. coli DNA polymerase I is the enzyme of choice for determining the ...
Page 13-62
... Klenow fragment of E. coli DNA polymerase I ( 6 units for each template sequence ; i.e. , 1.5 units for each of four sequencing reactions ) to the Klenow mixture prepared in step 5. Mix the contents of the tube thoroughly by gently ...
... Klenow fragment of E. coli DNA polymerase I ( 6 units for each template sequence ; i.e. , 1.5 units for each of four sequencing reactions ) to the Klenow mixture prepared in step 5. Mix the contents of the tube thoroughly by gently ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml