Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-46
... nucleic acid probes . In those rare instances when both types of reagents are available , nucleic acid probes are preferred because they can be used under a variety of different stringencies that minimize the chance of undesirable cross ...
... nucleic acid probes . In those rare instances when both types of reagents are available , nucleic acid probes are preferred because they can be used under a variety of different stringencies that minimize the chance of undesirable cross ...
Page 9-42
... nucleic acids are attached by hydrophobic rather than covalent interactions and are released slowly from the matrix during hybridization and washing at high temperatures . Second , nitrocellulose filters become brittle when dry and ...
... nucleic acids are attached by hydrophobic rather than covalent interactions and are released slowly from the matrix during hybridization and washing at high temperatures . Second , nitrocellulose filters become brittle when dry and ...
Page 9-61
... Nucleic Acids Res . 8 : 2453 . Nygaard , A.P. and B.D. Hall . 1963. A method for the detection of RNA - DNA complexes . Biochem . Biophys . Res . Commun . 12 : 98 . Olszewska , E. and K. Jones . 1988. Vac- uum blotting enhances nucleic ...
... Nucleic Acids Res . 8 : 2453 . Nygaard , A.P. and B.D. Hall . 1963. A method for the detection of RNA - DNA complexes . Biochem . Biophys . Res . Commun . 12 : 98 . Olszewska , E. and K. Jones . 1988. Vac- uum blotting enhances nucleic ...
Contents
SYNTHESIS OF THE FIRST STRAND OF cDNA 8 | 8-11 |
MOLECULAR CLONING OF DOUBLESTRANDED cDNA 8 | 8-21 |
8 | 8-29 |
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA fragments DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector