Molecular Cloning: A Laboratory Manual, Book 2 |
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Results 1-3 of 54
Page 9-39
... Place the inverted gel on the support so that it is centered on the wet 3MM papers . Make sure that there are no air bubbles between the 3MM paper and the gel . 8. Surround , but do not cover , the gel with Saran Wrap or Parafilm . This ...
... Place the inverted gel on the support so that it is centered on the wet 3MM papers . Make sure that there are no air bubbles between the 3MM paper and the gel . 8. Surround , but do not cover , the gel with Saran Wrap or Parafilm . This ...
Page 13-49
... place along the sides ( see Figure 13.8 ) . A couple of minute dabs of petroleum jelly help to keep the spacers in position during the next steps . Make sure there is no dust on the plates , and then lay the smaller ( or notched ) plate ...
... place along the sides ( see Figure 13.8 ) . A couple of minute dabs of petroleum jelly help to keep the spacers in position during the next steps . Make sure there is no dust on the plates , and then lay the smaller ( or notched ) plate ...
Page 13-56
... place the plate back on the gel , invert the plates , and try again . 3. When the glass plates have been separated , cut off the bottom corner of the side of the gel that was loaded first . This serves to orient the gel during the ...
... place the plate back on the gel , invert the plates , and try again . 3. When the glass plates have been separated , cut off the bottom corner of the side of the gel that was loaded first . This serves to orient the gel during the ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml