Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 13-8
... Sequenase and Sequenase version 2.0 Taq DNA polymerase and AmpliTaq с Processivity Rate of polymerization® 10-50 n.d. ~ 2000 ~ 3000 > 7600 45 5 300 35-100 Processivity is expressed as the average number of nucleotides synthesized before ...
... Sequenase and Sequenase version 2.0 Taq DNA polymerase and AmpliTaq с Processivity Rate of polymerization® 10-50 n.d. ~ 2000 ~ 3000 > 7600 45 5 300 35-100 Processivity is expressed as the average number of nucleotides synthesized before ...
Page 13-9
... Sequenase version 2.0 is a genetically engineered form of Sequenase that entirely lacks 3'5 ' exonuclease activity , is extremely stable , and has a threefold higher specific activity than the chemically modified enzyme . Sequenases are ...
... Sequenase version 2.0 is a genetically engineered form of Sequenase that entirely lacks 3'5 ' exonuclease activity , is extremely stable , and has a threefold higher specific activity than the chemically modified enzyme . Sequenases are ...
Page 15-64
... Sequenase ( Schena 1989 ) is used , the Klenow fragment generally gives yields of mutants that are more than adequate . However , if a single phosphorylated mutagenic oligonucleotide is used as a primer ( e.g. , in the Kunkel method of ...
... Sequenase ( Schena 1989 ) is used , the Klenow fragment generally gives yields of mutants that are more than adequate . However , if a single phosphorylated mutagenic oligonucleotide is used as a primer ( e.g. , in the Kunkel method of ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml