Molecular Cloning: A Laboratory Manual, Book 2 |
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... Smal Pstl Xbal Th111 Tth 1111 BstEll EcoRI Sacl In PSP64CS , the Hindill site in the polycloning site is downstream from the SP6 promoter . In PSP65CS , the EcoRI site in the polycloning site is downstream from the SP6 promoter . FIGURE ...
... Smal Pstl Xbal Th111 Tth 1111 BstEll EcoRI Sacl In PSP64CS , the Hindill site in the polycloning site is downstream from the SP6 promoter . In PSP65CS , the EcoRI site in the polycloning site is downstream from the SP6 promoter . FIGURE ...
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acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml