Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 11-19
... table contains a representative sample of the conditions used to screen mammalian cDNA libraries with oligonucleotides containing inosine . It was not our intention to present a listing of all genes that have been isolated in this way ...
... table contains a representative sample of the conditions used to screen mammalian cDNA libraries with oligonucleotides containing inosine . It was not our intention to present a listing of all genes that have been isolated in this way ...
Page 13-67
... Table 13.4 . TABLE 13.4 Modification of Sequenase Reactions to Optimize Sequencing of Different Lengths of DNA Distance of target DNA from 5 ' terminus of primer ( nucleotides ) 20-100 100-350 > 350 Modification Dilute the labeling ...
... Table 13.4 . TABLE 13.4 Modification of Sequenase Reactions to Optimize Sequencing of Different Lengths of DNA Distance of target DNA from 5 ' terminus of primer ( nucleotides ) 20-100 100-350 > 350 Modification Dilute the labeling ...
Page 13-83
... Table 13.6 . TABLE 13.6 Reagents , Solutions , and Apparatuses Required for DNA Sequencing 13.83 Preparation of Target DNA for Maxam-Gilbert Sequencing 13 83 Reagents, Solutions, and Apparatuses 13 83.
... Table 13.6 . TABLE 13.6 Reagents , Solutions , and Apparatuses Required for DNA Sequencing 13.83 Preparation of Target DNA for Maxam-Gilbert Sequencing 13 83 Reagents, Solutions, and Apparatuses 13 83.
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acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml