Molecular Cloning: A Laboratory Manual, Book 2 |
From inside the book
Results 1-3 of 92
Page 10-14
The purified DNA , mixed with a molar excess of primers , is denatured by boiling
, and synthesis is then carried out using the Klenow fragment of E . coli DNA
polymerase I . This enzyme lacks 5 ' → 3 ' exonuclease activity , so that the ...
The purified DNA , mixed with a molar excess of primers , is denatured by boiling
, and synthesis is then carried out using the Klenow fragment of E . coli DNA
polymerase I . This enzyme lacks 5 ' → 3 ' exonuclease activity , so that the ...
Page 10-43
The problem of having a limited quantity of absorbed probe with low specific
activity can be circumvented to a large extent by using subtracted probes
radiolabeled to high specific activity . SUBTRACTED PROBES RADIOLABELED
TO HIGH ...
The problem of having a limited quantity of absorbed probe with low specific
activity can be circumvented to a large extent by using subtracted probes
radiolabeled to high specific activity . SUBTRACTED PROBES RADIOLABELED
TO HIGH ...
Page 11-41
specific activities as high as 2 x 10 " ° cpm per microgram of probe . ... When
planning these sorts of reactions , it is important to consider the following points :
• The specific activity of the [ a - " P ] dNTPs in the reaction . The specific activity of
a ...
specific activities as high as 2 x 10 " ° cpm per microgram of probe . ... When
planning these sorts of reactions , it is important to consider the following points :
• The specific activity of the [ a - " P ] dNTPs in the reaction . The specific activity of
a ...
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Contents
CONCENTRATING NUCLEIC ACIDS E | 8-10 |
SYNTHESIS OF THE FIRST STRAND OF DNA 8 | 8-11 |
MEASUREMENT OF RADIOACTIVITY IN NUCLEIC ACIDS E | 8-18 |
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Common terms and phrases
aliquots allow amount amplification antibody appropriate approximately bacteriophage bacteriophage T4 base binding buffer carried cDNA cells centrifugation Chapter chromatography cleaved clones colonies complete concentration constructed containing deletions denatured described detection determine digestion dNTPs double-stranded efficiency electrophoresis et al ethanol expression extraction Figure filters four fragment gene genomic DNA hybridization increase Incubate inserted interest isolated Klenow labeled length libraries ligation linear linkers membrane method minutes mixture molecules mRNA mutants nucleic acids nucleotides obtained oligonucleotide plaques plasmid DNA plate position possible precipitation prepared presence primer probe problem protein purified radioactivity radiolabeled reaction recombinant region remove restriction enzyme room temperature samples screening separate sequence single single-stranded solution step stored strand synthesis target DNA target sequence template termini transfer Tris tube units usually vector volume