Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-21
... added to the double - stranded cDNA and complementary dG residues were added to a plasmid vector that had been digested with PstI ( Villa - Komaroff et al . 1978 ; Rowekamp and Firtel 1980 ) . This enzyme cleaves the sequence 5 ' CTGCAG ...
... added to the double - stranded cDNA and complementary dG residues were added to a plasmid vector that had been digested with PstI ( Villa - Komaroff et al . 1978 ; Rowekamp and Firtel 1980 ) . This enzyme cleaves the sequence 5 ' CTGCAG ...
Page 8-27
... added to the mRNA : cDNA hybrid , which is then annealed to a plasmid carrying dT tails . Because the efficiency of tailing the 3 ' - hydroxyl termini of RNA molecules is at least ten times less than that of the homologous reaction with ...
... added to the mRNA : cDNA hybrid , which is then annealed to a plasmid carrying dT tails . Because the efficiency of tailing the 3 ' - hydroxyl termini of RNA molecules is at least ten times less than that of the homologous reaction with ...
Page 10-51
... added to the reaction . Which of the four [ a - 32P ] dNTPs is added to the reaction depends on the sequence of the protruding 5 ' termini at the ends of the DNA . For example , ends created by cleavage of DNA with EcoRI can be labeled ...
... added to the reaction . Which of the four [ a - 32P ] dNTPs is added to the reaction depends on the sequence of the protruding 5 ' termini at the ends of the DNA . For example , ends created by cleavage of DNA with EcoRI can be labeled ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml