Molecular Cloning: A Laboratory Manual, Book 2 |
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Page 8-57
... aliquots at -70 ° C . Make a stock solution in water . Store in small aliquots at -20 ° C . Use strains C600 ( BNN93 ) for growth and BNN102 ( C600hflA ) for screening of cDNA libraries constructed in bacteriophage Agt10 . Use strain ...
... aliquots at -70 ° C . Make a stock solution in water . Store in small aliquots at -20 ° C . Use strains C600 ( BNN93 ) for growth and BNN102 ( C600hflA ) for screening of cDNA libraries constructed in bacteriophage Agt10 . Use strain ...
Page 8-66
... aliquots ( 2 μl each ) from the large - scale reaction and place in small microfuge tubes . Number these aliquots 3 and 4 . 5. To all four aliquots , add 100 ng of a plasmid such as Xf3 or pBR322 that has been digested to completion ...
... aliquots ( 2 μl each ) from the large - scale reaction and place in small microfuge tubes . Number these aliquots 3 and 4 . 5. To all four aliquots , add 100 ng of a plasmid such as Xf3 or pBR322 that has been digested to completion ...
Page 15-65
... aliquots of the undiluted reaction mixture and of each dilution of the reaction mixture with 200 - μl aliquots of competent TG1 cells ( prepared as described in Chapter 1 , pages 1.76-1.81 ) . c . Store the mixtures on ice for 30 ...
... aliquots of the undiluted reaction mixture and of each dilution of the reaction mixture with 200 - μl aliquots of competent TG1 cells ( prepared as described in Chapter 1 , pages 1.76-1.81 ) . c . Store the mixtures on ice for 30 ...
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Common terms and phrases
acrylamide agarose gel aliquots amplification antibody bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells centrifugation at 12,000g Chapter chromatography cleavage cleaved cleotide codon coli DNA polymerase complementary concentration containing ddNTPs deletions denatured described digestion diluted dithiothreitol DNA ligase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol exonuclease expression libraries formamide fusion proteins gel electrophoresis gene genomic DNA H₂O hybridization Klenow fragment labeled ligation linear linkers method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutants Natl nitrocellulose nitrocellulose filters nucleic acids nucleotides oligonucleotide phagemid plaques plasmid plasmid DNA plate polymerase chain reaction prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples Sequenase sequencing reactions solution specific activity step stored strand of cDNA synthesis target DNA target sequence template DNA termini transcription transfer Tris Cl pH vector µg/ml